No, the beds are made up pretty tight when fresh sheets are put on the bed. I think if they sat unoccupied for awhile, I would pull the quilts or coverlets and freshen those. I try to flip my rooms right after check-outs - I just like to have the rooms done and I do get calls for same day bookings. Today was a busy day though and I didn't get to vacuum two rooms. Tomorrow is another day.....
I check unoccupied rooms pretty much daily - when you live in an old house (even a restored one) and own a second one, you never know if there might be a surprise. Like today when I was flipping a room, I found dead Asian ladybugs way up high in the window weight cord and stuck to the wood blinds that the last person that cleaned the room (my part-time housekeeper) obviously missed. ARrrrrrrrgh!
Also the reason why I "ice" the rooms before check-ins. I thoroughly clean everything when a room is flipped and then hit the room with a Swiffer extendable duster (windows, blinds, furniture, etc.) and Swiffer sweeper (under beds especially checked) before check-ins. I also double check all the lights. We have been having an abundance of rain lately, so all the windows got opened and the "outside" sills got cleaned today on the rooms that I flipped. The other thing that we do here is have the room ready for the guest on arrival - a few lights on, TV turned on to the guide channel with the volume turned down, drapes (and/or blinds opened), snack basket and sweet treat in the room. That's part of our "icing" routine here..